SufR, a [4Fe-4S] Cluster-Containing Transcription Factor, Represses the sufRBDCSU Operon in Streptomyces avermitilis Iron-Sulfur Cluster Assembly.
Identifieur interne : 000030 ( Main/Exploration ); précédent : 000029; suivant : 000031SufR, a [4Fe-4S] Cluster-Containing Transcription Factor, Represses the sufRBDCSU Operon in Streptomyces avermitilis Iron-Sulfur Cluster Assembly.
Auteurs : Yaqing Cheng [République populaire de Chine] ; Mengya Lyu [République populaire de Chine] ; Renjun Yang [République populaire de Chine] ; Ying Wen [République populaire de Chine] ; Yuan Song [République populaire de Chine] ; Jilun Li [République populaire de Chine] ; Zhi Chen [République populaire de Chine]Source :
- Applied and environmental microbiology [ 1098-5336 ] ; 2020.
Abstract
Iron-sulfur (Fe-S) clusters are ubiquitous and versatile inorganic cofactors that are crucial for many fundamental bioprocesses in nearly all organisms. How cells maintain Fe-S cluster homeostasis is not well understood in Gram-positive bacteria. Genomic analysis showed that the Suf system, which is encoded by the sufRBDCSU operon, is the sole Fe-S cluster assembly system in the genus StreptomycesStreptomyces avermitilis is the industrial producer of avermectins, which are widely used as agricultural pesticides and antiparasitic agents. sufR (SAV6324) encodes a putative ArsR-family transcriptional regulator, which was characterized as a repressor of the sufRBDCSU operon in this investigation. Spectroscopy and mass spectrometry demonstrated that anaerobically isolated SufR contained an oxidation-sensitive [4Fe-4S] cluster and existed as a homodimer. Electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analyses revealed that [4Fe-4S]-SufR bound specifically and tightly to a 14-bp palindromic sequence (CAAC-N6-GTTG) in the promoter region of the sufR operon, repressing expression of the sufRBDCSU operon. The presence of the [4Fe-4S] cluster is critical for the DNA-binding activity of SufR. Cys182, Cys195, and Cys223 in the C-terminal region of SufR are essential for [4Fe-4S] cluster coordination, but Cys178 is not. The fourth non-Cys ligand in coordination of the [4Fe-4S] cluster for SufR remains to be identified. The findings clarify the transcriptional control of the suf operon by [4Fe-4S] SufR to satisfy the various Fe-S cluster demands. SufR senses the intracellular Fe-S cluster status and modulates the expression of the sole Fe-S cluster assembly system via its Fe-S cluster occupancy.IMPORTANCE Fe-S clusters function as cofactors of proteins controlling diverse biological processes, such as respiration, photosynthesis, nitrogen fixation, DNA replication, and gene regulation. The mechanism of how Actinobacteria regulate the expression of the sole Fe-S cluster assembly system in response to the various Fe-S cluster demands remains to be elucidated. In this study, we showed that SufR functions as a transcriptional repressor of the sole Fe-S cluster assembly system in the avermectin producer S. avermitilis [4Fe-4S]-SufR binds to the promoter region of the suf operon and represses its expression. When Fe-S cluster levels are insufficient, SufR loses its [4Fe-4S] cluster and DNA-binding activity. Apo-SufR dissociates from the promoter region of suf operon, and the expression of the suf system is strongly increased by derepression to promote the synthesis of Fe-S clusters. The study clarifies how Streptomyces maintains its Fe-S cluster homeostasis through the activity of SufR to modulate the various Fe-S cluster demands.
DOI: 10.1128/AEM.01523-20
PubMed: 32680866
PubMed Central: PMC7480368
Affiliations:
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China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Jilun" sort="Li, Jilun" uniqKey="Li J" first="Jilun" last="Li">Jilun Li</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Zhi" sort="Chen, Zhi" uniqKey="Chen Z" first="Zhi" last="Chen">Zhi Chen</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China chenzhi@cau.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, 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uniqKey="Cheng Y" first="Yaqing" last="Cheng">Yaqing Cheng</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lyu, Mengya" sort="Lyu, Mengya" uniqKey="Lyu M" first="Mengya" last="Lyu">Mengya Lyu</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Renjun" sort="Yang, Renjun" uniqKey="Yang R" first="Renjun" last="Yang">Renjun Yang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wen, Ying" sort="Wen, Ying" uniqKey="Wen Y" first="Ying" last="Wen">Ying Wen</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Song, Yuan" sort="Song, Yuan" uniqKey="Song Y" first="Yuan" last="Song">Yuan Song</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Jilun" sort="Li, Jilun" uniqKey="Li J" first="Jilun" last="Li">Jilun Li</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Zhi" sort="Chen, Zhi" uniqKey="Chen Z" first="Zhi" last="Chen">Zhi Chen</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China chenzhi@cau.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="eISSN">1098-5336</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Iron-sulfur (Fe-S) clusters are ubiquitous and versatile inorganic cofactors that are crucial for many fundamental bioprocesses in nearly all organisms. How cells maintain Fe-S cluster homeostasis is not well understood in Gram-positive bacteria. Genomic analysis showed that the Suf system, which is encoded by the <i>sufRBDCSU</i> operon, is the sole Fe-S cluster assembly system in the genus <i>Streptomyces</i><i>Streptomyces avermitilis</i> is the industrial producer of avermectins, which are widely used as agricultural pesticides and antiparasitic agents. <i>sufR</i> (<i>SAV6324</i>) encodes a putative ArsR-family transcriptional regulator, which was characterized as a repressor of the <i>sufRBDCSU</i> operon in this investigation. Spectroscopy and mass spectrometry demonstrated that anaerobically isolated SufR contained an oxidation-sensitive [4Fe-4S] cluster and existed as a homodimer. Electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analyses revealed that [4Fe-4S]-SufR bound specifically and tightly to a 14-bp palindromic sequence (CAAC-N6-GTTG) in the promoter region of the <i>sufR</i> operon, repressing expression of the <i>sufRBDCSU</i> operon. The presence of the [4Fe-4S] cluster is critical for the DNA-binding activity of SufR. Cys<sup>182</sup>, Cys<sup>195</sup>, and Cys<sup>223</sup> in the C-terminal region of SufR are essential for [4Fe-4S] cluster coordination, but Cys<sup>178</sup> is not. The fourth non-Cys ligand in coordination of the [4Fe-4S] cluster for SufR remains to be identified. The findings clarify the transcriptional control of the <i>suf</i> operon by [4Fe-4S] SufR to satisfy the various Fe-S cluster demands. SufR senses the intracellular Fe-S cluster status and modulates the expression of the sole Fe-S cluster assembly system via its Fe-S cluster occupancy.<b>IMPORTANCE</b> Fe-S clusters function as cofactors of proteins controlling diverse biological processes, such as respiration, photosynthesis, nitrogen fixation, DNA replication, and gene regulation. The mechanism of how <i>Actinobacteria</i> regulate the expression of the sole Fe-S cluster assembly system in response to the various Fe-S cluster demands remains to be elucidated. In this study, we showed that SufR functions as a transcriptional repressor of the sole Fe-S cluster assembly system in the avermectin producer <i>S. avermitilis</i> [4Fe-4S]-SufR binds to the promoter region of the <i>suf</i> operon and represses its expression. When Fe-S cluster levels are insufficient, SufR loses its [4Fe-4S] cluster and DNA-binding activity. Apo-SufR dissociates from the promoter region of <i>suf</i> operon, and the expression of the <i>suf</i> system is strongly increased by derepression to promote the synthesis of Fe-S clusters. The study clarifies how <i>Streptomyces</i> maintains its Fe-S cluster homeostasis through the activity of SufR to modulate the various Fe-S cluster demands.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32680866</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>27</Day></DateRevised><Article PubModel="Electronic-Print"><Journal><ISSN IssnType="Electronic">1098-5336</ISSN><JournalIssue CitedMedium="Internet"><Volume>86</Volume><Issue>18</Issue><PubDate><Year>2020</Year><Month>09</Month><Day>01</Day></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>SufR, a [4Fe-4S] Cluster-Containing Transcription Factor, Represses the <i>sufRBDCSU</i> Operon in Streptomyces avermitilis Iron-Sulfur Cluster Assembly.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">e01523-20</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1128/AEM.01523-20</ELocationID><Abstract><AbstractText>Iron-sulfur (Fe-S) clusters are ubiquitous and versatile inorganic cofactors that are crucial for many fundamental bioprocesses in nearly all organisms. How cells maintain Fe-S cluster homeostasis is not well understood in Gram-positive bacteria. Genomic analysis showed that the Suf system, which is encoded by the <i>sufRBDCSU</i> operon, is the sole Fe-S cluster assembly system in the genus <i>Streptomyces</i><i>Streptomyces avermitilis</i> is the industrial producer of avermectins, which are widely used as agricultural pesticides and antiparasitic agents. <i>sufR</i> (<i>SAV6324</i>) encodes a putative ArsR-family transcriptional regulator, which was characterized as a repressor of the <i>sufRBDCSU</i> operon in this investigation. Spectroscopy and mass spectrometry demonstrated that anaerobically isolated SufR contained an oxidation-sensitive [4Fe-4S] cluster and existed as a homodimer. Electrophoretic mobility shift assays (EMSAs) and DNase I footprinting analyses revealed that [4Fe-4S]-SufR bound specifically and tightly to a 14-bp palindromic sequence (CAAC-N6-GTTG) in the promoter region of the <i>sufR</i> operon, repressing expression of the <i>sufRBDCSU</i> operon. The presence of the [4Fe-4S] cluster is critical for the DNA-binding activity of SufR. Cys<sup>182</sup>, Cys<sup>195</sup>, and Cys<sup>223</sup> in the C-terminal region of SufR are essential for [4Fe-4S] cluster coordination, but Cys<sup>178</sup> is not. The fourth non-Cys ligand in coordination of the [4Fe-4S] cluster for SufR remains to be identified. The findings clarify the transcriptional control of the <i>suf</i> operon by [4Fe-4S] SufR to satisfy the various Fe-S cluster demands. SufR senses the intracellular Fe-S cluster status and modulates the expression of the sole Fe-S cluster assembly system via its Fe-S cluster occupancy.<b>IMPORTANCE</b> Fe-S clusters function as cofactors of proteins controlling diverse biological processes, such as respiration, photosynthesis, nitrogen fixation, DNA replication, and gene regulation. The mechanism of how <i>Actinobacteria</i> regulate the expression of the sole Fe-S cluster assembly system in response to the various Fe-S cluster demands remains to be elucidated. In this study, we showed that SufR functions as a transcriptional repressor of the sole Fe-S cluster assembly system in the avermectin producer <i>S. avermitilis</i> [4Fe-4S]-SufR binds to the promoter region of the <i>suf</i> operon and represses its expression. When Fe-S cluster levels are insufficient, SufR loses its [4Fe-4S] cluster and DNA-binding activity. Apo-SufR dissociates from the promoter region of <i>suf</i> operon, and the expression of the <i>suf</i> system is strongly increased by derepression to promote the synthesis of Fe-S clusters. The study clarifies how <i>Streptomyces</i> maintains its Fe-S cluster homeostasis through the activity of SufR to modulate the various Fe-S cluster demands.</AbstractText><CopyrightInformation>Copyright © 2020 American Society for Microbiology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Yaqing</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lyu</LastName><ForeName>Mengya</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Renjun</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Yuan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jilun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zhi</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China chenzhi@cau.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Fe-S cluster homeostasis</Keyword><Keyword MajorTopicYN="Y">S. avermitilis</Keyword><Keyword MajorTopicYN="Y">SufR</Keyword><Keyword MajorTopicYN="Y">[4Fe-4S] cluster</Keyword><Keyword MajorTopicYN="Y">suf operon</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>06</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pmc-release"><Year>2021</Year><Month>03</Month><Day>01</Day></PubMedPubDate><PubMedPubDate 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Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Cheng, Yaqing" sort="Cheng, Yaqing" uniqKey="Cheng Y" first="Yaqing" last="Cheng">Yaqing Cheng</name></noRegion><name sortKey="Chen, Zhi" sort="Chen, Zhi" uniqKey="Chen Z" first="Zhi" last="Chen">Zhi Chen</name><name sortKey="Chen, Zhi" sort="Chen, Zhi" uniqKey="Chen Z" first="Zhi" last="Chen">Zhi Chen</name><name sortKey="Cheng, Yaqing" sort="Cheng, Yaqing" uniqKey="Cheng Y" first="Yaqing" last="Cheng">Yaqing Cheng</name><name sortKey="Li, Jilun" sort="Li, Jilun" uniqKey="Li J" first="Jilun" last="Li">Jilun Li</name><name sortKey="Li, Jilun" sort="Li, Jilun" uniqKey="Li J" first="Jilun" last="Li">Jilun Li</name><name sortKey="Lyu, Mengya" sort="Lyu, Mengya" uniqKey="Lyu M" first="Mengya" last="Lyu">Mengya Lyu</name><name sortKey="Lyu, Mengya" sort="Lyu, Mengya" uniqKey="Lyu M" first="Mengya" last="Lyu">Mengya Lyu</name><name sortKey="Song, Yuan" sort="Song, Yuan" uniqKey="Song Y" first="Yuan" last="Song">Yuan Song</name><name sortKey="Song, Yuan" sort="Song, Yuan" uniqKey="Song Y" first="Yuan" last="Song">Yuan Song</name><name sortKey="Wen, Ying" sort="Wen, Ying" uniqKey="Wen Y" first="Ying" last="Wen">Ying Wen</name><name sortKey="Wen, Ying" sort="Wen, Ying" uniqKey="Wen Y" first="Ying" last="Wen">Ying Wen</name><name sortKey="Yang, Renjun" sort="Yang, Renjun" uniqKey="Yang R" first="Renjun" last="Yang">Renjun Yang</name><name sortKey="Yang, Renjun" sort="Yang, Renjun" uniqKey="Yang R" first="Renjun" last="Yang">Renjun Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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